Point-of-care (POC) biosensors are integrated diagnostic systems employed for the detection of clinically relevant analytes in biological fluids such as blood, urine and saliva. These devices offer the advantage to provide rapid results directly where the information is needed (e.g. patient’s home, doctor’s office or emergency room), thus facilitating an earlier diagnosis and a prompt patient’s treatment. Various technologies have been proposed for the realization of POC biosensors including label-free techniques based on optical, mechanical and electrochemical transducers. However, reliable, quantitative and ultrasensitive devices have been not yet commercialized. Electronic biosensors based on organic thin-film transistors (OTFTs) are a promising choice for the development of the next generation of POC devices. These biosensors can be combined with integrated electrical circuits, microfluidic systems and wireless technologies. Furthermore, they offer high sensitivity, biocompatibility and possibility to produce all-printed low-cost biosensors in flexible and disposable formats. Among them, electrolyte-gated (EG)-OTFTs have been identified as ideal candidates for biosensors development as they operate at low voltages directly in aqueous buffer solutions. Using these configurations ultrasensitive label-free immunosensors for the detection of C-reactive protein (CRP), a specific biomarker of inflammatory and infection diseases, at the femtomolar concentration level have been developed. The devices are also able to perform chiral differential detection of odorant molecules. The specific features of the proposed EGOTFT biosensors as well as their analytical performances will be discussed.

- L. Torsi, M. Magliulo, K. Manoli, G. Palazzo, Chemical Society Review 42 (2013) 8612-8628.
- M.Y. Mulla, E. Tuccori, M. Magliulo, G. Lattanzi, G. Palazzo, K. Persaud and L. Torsi. Nature Communications 6 (2015) 6010.
- K. Manoli et al. Angewandte Chemie International Edition, 2015, 54, 12562–12576.